Genotyping tools and resources to assess peanut germplasm: smut-resistant landraces as a case study

Peanut smut caused by Thecaphora frezii is a severe fungal disease currently endemic to Argentina and Brazil. The identification of resistant germplasm crucial in view the potential risk global spread. In recent study, we reported new sources resistance demonstrated its introgression into elite peanut cultivars. Here, revisited one these (line I0322) verify presence U.S. collection identify single nucleotide polymorphisms (SNPs) potentially associated with resistance. Five accessions Arachis hypogaea subsp. fastigiata from collection, along source derived inbred lines were genotyped 48K SNP array. A recently developed genotyping platform called RNase H2 enzyme-based amplification (rhAmp) was further applied validate selected SNPs larger number individuals per accession. More than 14,000 nine rhAmp assays confirmed that 98.6% identical ( P < 0.01, bootstrap t -test) line I0322. We report this accompanying genetic information, data, diagnostic markers.